komponisto comments on Experiment: Knox case debate with Rolf Nelson - Less Wrong
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We're talking here not about the time it takes for the stomach to emtpy completely, but rather the time it takes for ingesta to begin passing into the duodenum ("T_lag"). (At death, there was 500mL of ingesta in the stomach -- consistent with the meal size -- and nothing in the duodenum.) According to this paper, the median value of T_lag is 81.5 minutes, and the 75th percentile is 102 minutes. (Furthermore, the median time for half the contents to empty ["T_1/2"] is 127 minutes, and the 75th percentile is 168.3 minutes.) The prosecution scenario of death during the 11:00 pm hour would require a T_lag of more than 240 minutes, possibly more than 300. My understanding is that this is basically unheard of, whatever the composition of the meal.
Ronchi claimed that the coroner, Lalli, had failed to seal the duodenum via ligature, as is apparently the standard procedure; this was the basis for his claim that food could have slipped into the small intestine. However, video of the autopsy revealed that Ronchi was wrong, and that Lalli had indeed properly sealed the duodenum. (Sollecito appeal, p. 165)
There were four samples on the blade, B,C,E, and G that were tested for blood; the results were all negative. These traces were all presumed to be blood by Stefanoni; it seems reasonable to suppose that if there had been blood on the knife, these would have been the most likely spots in which to have found it.
A positive blood test result would require more than DNA from (white) blood cells -- it would require hemoglobin. So it seems to me that the only way to get a positive blood test result from contamination would be to spill a blood sample on the knife. I estimate the probability of this having happened as being in the range of 0.001.
On the other hand, in the event that the knife had been used for stabbing, and that the victim's DNA remained on the knife, I would estimate a probability northward of 0.9 that at least one of the "presumed blood" traces would have tested positive for blood. (Cf. ChrisHalkides' comment below: "Two experts have publicly stated that the chances of cleaning a bloody knife to the point at which blood is no longer detected but DNA is detected, are small." This agrees with my intuition, and "no more than 0.1" seems a reasonable interpretation of "small".)
It's worse than that; what Conti and Vecchiotti describe is suggestive of deliberate misrepresentation if not outright fabrication of results. The "several hundred picograms" quantification result for Trace B appears to have been completely made up. She claimed in court that she had obtained this result using Real Time PCR, but this was not the case: records obtained by Conti and Vecchiotti show that another method ("Qubit Fluorometer") was used, and that the result obtained was "too low" -- the exact same as for Trace C. There was no justification for treating Trace B as a positive result and Trace C as a negative, and in particular no reason for subjecting Trace B to amplification.
Yes (p.61).
This is on p. 79. This section of the report, on Stefanoni's knife results, is next in line to be translated (by my collaborator katy_did, while I'll be simultaneuously doing the clasp section).
Substantially different. Stefanoni is quoted as follows on p. 102 of the report:
"...the knife was analyzed...in the course of these 50 samples attributed to the victim, some were prior to the analysis of the knife, of course, and others subsequent, so in these 50 I don't know if the knife was, I don't know now, a fourth, a third of the way through this flux of analyses..."
This puts me at 0.5 or more. Also note that the number 50 itself gives a probability of 0.1 if we use your estimate of 500 total samples in the lab.
Let's talk about the dna some more once you guys have finished translating the relevant parts of the independent report, then, if your argument hinges on details of the independent report rather than just the conclusions.
Sounds good. In your case, for one particular meal where the subjects had probably fasted beforehand, the lag is just under 2/3 of the half-time. If you accept Umani Rochi's half-time of 360-420 minutes, then the lag could be 2/3 of that, or 240+ minutes. Of course, for all I know Umani Rochi could have been referring to the lag time, or the final gastric emptying time, rather than the half-time. Lags could easily be much smaller, or larger, than 2/3 of the half-time in this case.
It sounds like you might disagree with not just with Umani Rochi (a court-appointed expert), and Raffaele's consultant Vinci, but also with another of Raffaele's consultants, Introna, who placed the start of attack between 21:30 and 22:30.
Note that stress (such as being attacked) can increase lag time, so we might be talking about the time the attack started rather than the time of death.
In addition to the starchiness of the meal, I would claim that:
Alcohol (or drug use) may increase lag time, studies differ as to how significant this is though.
Subjects in studies usually fast before the study, which means in the real world I expect lag times to be longer. Meredith also returned home after the meal, which may be more physical activity than the subjects did, though I could be wrong about that.
Subjects in studies don't usually go and eat a snack after the meal, as I believe Meredith did. I would expect this to also increase Meredith's lag time.
Anyway, what's your model here: What do you personally estimate the lag to be based solely on digestion (assuming no slippage)? Maybe you can give a mean and a standard deviation, and we can start by modeling it as a normal distribution?
How much does application of the ligatures reduce the probability of slippage? If ligatures were not applied, how likely do you think complete slippage would be? If they are applied, what are the odds that (1) the slippage occurs before the ligatures are applied, or (2) the slippage occurs anyway after the ligatures are applied, perhaps due to improper application?
The translation is now nearing completion (the clasp section is finished, and the knife section will be soon). Here, furthermore, are some relevant links:
Great, give me a top-level post when the knife translation is finished, or when you think it's in a good enough state to back up your claims in the dna discussion.