rseiter comments on Get genotyped for free ( If your IQ is high enough) - Less Wrong

34 Post author: wallowinmaya 01 October 2011 04:00PM

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Comment author: rseiter 05 April 2015 04:42:35PM 2 points [-]

Thanks for the explanation and tips! I used your procedure and ended up with the same 131MB file. Interestingly I did not need to remove the "--" entries. I have been exchanging email with BGI and they indicated files could have significantly different number of entries (but I am surprised at >3x!). Is there any chance your sequencing had greater than 4x coverage? My VCF file is queued up and should be available in a few months which should help clarify what I am seeing.

Comment author: VincentYu 07 April 2015 09:50:41AM 0 points [-]

Is there any chance your sequencing had greater than 4x coverage?

I don't know. How do I find out?

Comment author: rseiter 18 April 2015 01:17:48AM 0 points [-]

I think the VCF would tell you if you had it. Another possibility would be using a lower quality threshold for calling SNPs, but that seems unlikely.