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dekelron comments on Neuroscience basics for LessWrongians - Less Wrong
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I'm having trouble working out the experimental conditions here. I take it they replaced a sequence of zebrafish DNA with its human equivalent, which seemed to have been undergoing nearly neutral selection, and didn't observe developmental defects. But what was the condition where they did observe defects? If they just removed that section of DNA, that could suggest that some sequence is needed there but its contents are irrelevant. If they replaced it with a completely different section of DNA that seems like it would be a lot more surprising.
The DNA in the zebrafish was deleted, and the human version was inserted later, without affecting the main DNA (probably using a "plasmid"). Without the human DNA "insert", there was a developmental defect. with either the human DNA insert or the original zebrafish DNA (as an insert), there was no developmental defect, leading to the conclusion that the human version is functionally equivalent to the zebrafish version.
How do we know whether, by replacing the insert with a random sequence of base pairs the same length, there would be no developmental defect either?
There are several complications addressed in the article, which I did not describe. Anyway, using a "control vector" is considered trivial, and I believe they checked this.