After two days, a discussion will die down to the point where it barely gets any responses. If you're going to make it ladies only, make it ladies only permanently. Make an identical male only counterpart. That would solve the problem "Where will the men post?" and give you a nice undiluted control for your observations about the women. It will also help keep things organized (Otherwise can you imagine the overhead in going through the thread trying to figure out who was male and who was female, and reading each time stamp to determine who was who? It's much much easier to make two threads.) If women know that men will reply to their comments later, this may inhibit them from saying certain things the same way that it will inhibit them if men are there right away. If they know the men are never supposed to reply to that comment, that would help maximize the women's comfort.