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If I email someone non-famous to be on my podcast and they don't respond should I take that as a "no" or as a "didn't get the message try again".
I make no claim to know what you should do, but what I would do in that situation is: wait at least a month; then email them again saying: no obligation to reply or anything, just wanted to make sure emails aren't going astray. If still no reply, assume that either they aren't interested or they're spam-binning your emails and you'll never reach them by email anyway.