RobertLumley comments on How would you take over Rome? - Less Wrong Discussion
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Assuming my diseases don't automatically kill everyone I come in contact with:
Their folk beliefs about diseases (diseases are caused by evil spirits that can spread from person to person) are close enough to the truth that his probably wouldn't work. Also they believe in witchcraft and would notice if all your enemies suddenly started getting sick.
As far as #4 goes, there are plenty of classic simple experiments - Pasteur's disproof of spontaneous generation and the demonstration of oxygen/carbon dioxide (mice and plants in bell jars) come to mind.