Most of the sensible people seem to be saying that the relevant neural features can be observed at a 5nm x 5nm x 5nm spatial resolution, if supplemented with some gross immunostaining to record specific gene expressions and chemical concentrations. We already have SEM setups that can scan vitrified tissue at around that resolution, they're just (several) orders of magnitude too slow. Outfitting them to do immunostaining and optical scanning would be relatively trivial. Since multi-beam SEMS are expected to dramatically increase the scan rate in the next couple of years, and since you could get excellent economies of scale for scanning on parallel machines, I do not expect the scanners themselves to be the bottleneck technology.
The other possible bottleneck is the actual neuroscience, since we've got a number of blind spots in the details of how large-scale neural machinery operates. We don't know all the factors we would need to stain for, we don't know all of the details of how synaptic morphology correlates with statistical behavior, and we don't know how much detail we need in our neural models to preserve the integrity of the whole (though we have some solid guesses). We also do not, to the best of my knowledge, have reliable computational models of glial cells at this point. There are also a few factors of questionable importance, like passive neurotransmitter diffusion and electrical induction that need further study to decide how (if at all) to account for them in our models. However, progress in this area is very rapid. The Blue Brain project alone has made extremely strong progress in just a few years. I would be surprised if it took more than fifteen years to solve the remaining open questions.
Large scale image processing and data analytics, for parsing the scan images, is a sufficiently mature science that it's not my primary point of concern. What could really screw it up is if Moore's law craps out in ten years like Gordon Moore has predicted, and none of the replacement technologies are advanced enough to pick up the slack.
I heard that women are difficult to convince when it comes to signing up for cryo. In mentioning cryonics to a dying person, there seems to be a consensus that it's not going to happen. I encountered a post: Years saved: Cryonics vs VillageReach, which addressed my main objection (that the amount of money spent on cryo may be better spent on saving starving children, especially considering that you could save multiple children for that amount of money with high probability whereas you save only one life with low probability by paying for cryo). Now I'm open to being persuaded.
My first instinct was to go read a lot about cryo, but it dawned on me that there are a lot of people here who will want to convince family members, some of them female, to sign up - and these people may appreciate the opportunity to practice on somebody. It has been argued that "Brilliant and creative minds have explored the argument territory quite thoroughly." but if we already know all of the objections and have working rebuttals for each, why is it still thought of as extra difficult to get through to women? If there were a solution to this, it would not be seen as difficult. There must be something that pro-cryo people need for persuading women that they either haven't figured out or aren't good enough at yet.
So, I decided to offer myself for experiments in attempting to convince a woman to sign up for cryo and took a poll in an open thread to see whether there was interest. I don't claim to be perfectly representative of the female population, but I assume that I will have at least some objections in common with them and that persuading me would still be good practice for anyone planning to convince family members in the future. Having a study on persuading women would be more scientific but how do you come up with hypotheses to test for such a study if you have no actual experience persuading women?
So, here is your opportunity to try whatever methods of persuasion you feel like with no guilt, explore my full list of objections without worrying about it being socially awkward, (I will even share cached religious thoughts, as annoyed as I am that I still have them.), and I will document as many of my impressions and objections as I can before I forget them.
I am putting each objection / impression into a new comment for organization. Also, I have decided to avoid reading anything further on cryo, until/unless it is suggested by one of my persuaders.
Well, have fun getting inside my head.