Hello folks! I'm the person who presented the 'expansion x-ray microtomography' approach at the Oxford workshop. I wanted to mention that since that presentation, I've done a lot of additional research into what would be needed to facilitate 1 year imaging of human brain via synchrotron + ExM. I now have estimates for 11-fold expansion to 27 nm voxel size, which is much closer to what is needed for traceability (further expansion could also help if needed). You can find my updated proposal here: https://logancollinsblog.com/2023/02/22/feasibility-of-mapping-the-human-brain-with-expansion-x-ray-microscopy/
In addition, I am currently compiling improved estimates for the speed of other imaging modalities for comparison. I've done a deep dive into the literature and identified the most competitive approaches now available for electron microscopy (EM) and expansion light-sheet fluorescence microscopy (ExLSFM). I expect to make these numbers available in the near future, but I want to go through and revise my analysis paper before posting it. If you are interested in learning more early, feel free to reach out to me!
Hello folks! I'm the person who presented the 'expansion x-ray microtomography' approach at the Oxford workshop. I wanted to mention that since that presentation, I've done a lot of additional research into what would be needed to facilitate 1 year imaging of human brain via synchrotron + ExM. I now have estimates for 11-fold expansion to 27 nm voxel size, which is much closer to what is needed for traceability (further expansion could also help if needed). You can find my updated proposal here: https://logancollinsblog.com/2023/02/22/feasibility-of-mapping-the-human-brain-with-expansion-x-ray-microscopy/
In addition, I am currently compiling improved estimates for the speed of other imaging modalities for comparison. I've done a deep dive into the literature and identified the most competitive approaches now available for electron microscopy (EM) and expansion light-sheet fluorescence microscopy (ExLSFM). I expect to make these numbers available in the near future, but I want to go through and revise my analysis paper before posting it. If you are interested in learning more early, feel free to reach out to me!