We seem to have very different assumptions here. I am assuming you can get up to the molecule and gently wave a tiny molecular probe in its direction, if required. I am not assuming that you are trying to use high-energy photons to photograph it.
How are you imaging this works? I'm aware of chemistry that would allow you to say there are X whatever proteins, and Y such-and-such enzymes,etc, but such chemical processes I don't think are good enough for the sort of geometric reconstruction needed. Its not obvious to me that a molecular probe of the type you imagine can exist. What exactly is it measuring and how is it sensitive to it? Is it some sort of enzyme? Do we thaw the brain and then introduce these probes in solution? Do we somehow pulp the cell and run the constituents through a nanopore type thing and try to measure charge?
the intuitions of other scientists with domain knowledge of neuroscience who use the language of information theory when assessing cryonic feasibility.
I would love to be convinced I am overly pessimistic, and pointing me in the direction of biochemists/neuroscientists/biophysicists who disagree with me would be welcome. I only know a few biophysicists and they are generally more pessimistic than I am.
What does the vitrification solution actually do that's supposed to irreversibly map things, does anyone actually know?
I know ethylene glycol is cytotoxic, and so interacts with membrane proteins, but I don't know the mechanism.
I'll quickly point you at Drexler's Nanosystems and Freitas's Nanomedicine though they're rather long and technical reads. But we are visualizing molecularly specified machines, and 'hell no' to thawing first or pulping the cell. Seriously, this kind of background assumption is why I have to ask a lot of questions instead of just taking this sort of skeptical intuition at face value.
But rather than having to read through either of those sources, I would ask you to just take on assumption that two molecularly distinct (up to thermal noise) configurations ...
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