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A list of some posts that are pretty awesome
I recommend the major sequences to everybody, but I realize how daunting they look at first. So for purposes of immediate gratification, the following posts are particularly interesting/illuminating/provocative and don't require any previous reading:
- Your Intuitions are Not Magic
- The Apologist and the Revolutionary
- How to Convince Me that 2 + 2 = 3
- Lawful Uncertainty
- The Planning Fallacy
- Scope Insensitivity
- The Allais Paradox (with two followups)
- We Change Our Minds Less Often Than We Think
- The Least Convenient Possible World
- The Third Alternative
- The Domain of Your Utility Function
- Newcomb's Problem and Regret of Rationality
- The True Prisoner's Dilemma
- The Tragedy of Group Selectionism
- Policy Debates Should Not Appear One-Sided
- That Alien Message
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This has some problems- fundamentally the length scale probed is inversely proportional to the energy required, which means increasing the resolution increases the damage done by scanning. You start getting into issues of 'how much of this can I scan before I've totally destroyed this?' which is a sort of percolation problem (how many amino acids can I randomly knock out of a protein before it collapses or rebonds into a different protein?), so scanning at resolutions with energy equivalent above peptide bonds is very problematic. Assuming peptide bond strength of a couple kj/mol, I get lower-limit length scales of a few microns (this is rough, and I'd appreciate if someone would double check).
The vitrification solutions currently used are know to be toxic, and are used at very high concentrations, so some of this sort of damage will occur. I don't know enough biochemistry to say anything else with any kind of definitety, but on the previous thread kalla724 seemed to have some domain specific knowledge and thought the problem would be severe.
No, not at all. The vitrification damage is orders of magnitude less. Destroying a few multi-unit proteins and removing some inhibitors seems much better than totally destroying the cell-membrane (which has many of the same "which sites were these guys attached to?" problems).
Its my (limited) understanding that the cell membrane exist to largely solve this problem. Also, introducing tiny bits of toxins here and there causes small amounts of damage but the cell could probably survive. Putting the cell in a toxic environment will inevitably kill it. The concentration matters. But here I'm stepping way outside anything I know about.
We seem to have very different assumptions here. I am assuming you can get up to the molecule and gently wave a tiny molecular probe in its direction, if required. I am not assuming that you are trying to use high-energy photons to photograph it.
You also still seem to be use a lot of functional-damage words like "destroying" which is why I don't trust your... (read more)